Efficient gene targeted random mutagenesis in genetically stable Escherichia coli strains
نویسندگان
چکیده
منابع مشابه
Efficient gene targeted random mutagenesis in genetically stable Escherichia coli strains.
We describe a method to generate in vivo collections of mutants orders of magnitude larger than previously possible. The method favors accumulation of mutations in the target gene, rather than in the host chromosome. This is achieved by propagating the target gene on a plasmid, in Escherichia coli cells, within the region preferentially replicated by DNA polymerase I (Pol I), which replicates o...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2000
ISSN: 1362-4962
DOI: 10.1093/nar/28.21.e95